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video tang ck iu

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video tang ck iu

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We compared CK activity spectrophotometrically in tissues with high and fluctuating energy demands including cerebrum, cerebellum, heart, renal artery, and skeletal muscle, obtained post-mortem in black and white men. We used mixed linear regression models to assess the possible influence of ethnicity on CK activity in different tissues, with ethnicity as a fixed categorical subject factor, and CK of different tissues clustered within one person as the repeated effect response variable.

We collected post-mortem tissue samples from 17 white and 10 black males, mean age 62 y SE 4. We found evidence that black people have higher CK activity in all tissues with high and fluctuating energy demands studied.

This finding may help explain the higher serum CK levels found in this population subgroup. Furthermore, our data imply that there are differences in CK-dependent ATP buffer capacity in tissue between the black and the white population subgroup, which may become apparent with high energy demands. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

video tang ck iu

Funding: The Jan Dekker and Dr. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist. Creatine kinase CK activity in serum is widely used to diagnose tissue damage including myocardial infarction and skeletal muscle myopathy, but it is unknown why serum CK activity is higher in apparently healthy black people of sub-Saharan African descent [1] — [4].

There is no evidence of muscle damage in black people as cause for the high serum CK activity, and the BB, MB, and MM isoenzymes in serum are proportionally higher, but have a normal distribution [2][4]. Therefore, it was proposed that the black population subgroup has a generalized high CK activity in tissues, but hitherto there were no data to substantiate this [3].

In this paper, we report ethnic differences in CK activity in tissues with high and fluctuating energy demands. Tissues were collected in accordance with ethical approval of the accredited Medical Ethics Committee of the Academic Medical Center, Amsterdam, the Netherlands, whereby post-mortem small sample tissue collection was permitted with written consent from the donor, or a legal representative next-of-kin or other authorized representative. We only included tissues of subjects presented within 30 h after death.

Exclusion criteria included known HIV, hepatitis B, or C infection; Creutzfeldt-Jacob disease; chronic heart failure; myocardial infarction; brain infarction; or any widespread disease of the sampled organs.

Classification in black of sub-Saharan Africa or of sub-Saharan African descent, or white European was final when consensus was reached by at least two health workers pathologists, a member of the study group, or pathology assistants. Around 1 cubic centimeter of macroscopically healthy cerebral cortex gyrus precentraliscerebellar cortex, left cardiac ventricle, renal artery, and iliopsoas muscle was sampled.

The investigator was blinded for the ethnicity of the subjects. CK activity was determined according to Scholte et al. The baseline reaction rate was spectrophotometrically assessed Cary 1E Spectrophotometer; Varian Inc. The reaction rate was corrected for the baseline rate, and CK activity was calculated using a molar extinction coefficient of NADH of 6. The primary outcome was a difference in mean tissue creatine kinase activity between black and white people. Correlations with tissue CK activity were calculated using Pearson's correlations coefficients, for storage time, ethnicity, and for age nested within ethnicity.

Taking ethnic differences in the mean tissue CK of different tissues clustered within one person as the response variable in mixed linear regression modelling rather than testing pairs of tissue types separately with a Student t testis just, as we assumed the outcomes for tissue types within one person to be highly correlated.

In addition, we hereby greatly reduced the required sample size, which was of vital importance to be able to perform this type of study, as in our country, subject infrequently opt to donate post mortem tissue samples for scientific studies. Age was nested within ethnicity in the model. For the final model, we examined 3 covariance structures diagonal, autoregressive, and compound symmetryusing Akaike's Information Criterion and Schwarz's Bayesian Information Criterion.

A one-tailed p value of 0. We included 27 subjects, 10 black men of African-Caribbean descent and 17 white men, with a mean age of 62 y SE 4. Tissues were collected within a mean of 26 h SE 3. There was no significant correlation between storage time and mean tissue CK activity, nor between age and CK activity. We did find a significant correlation between CK and ethnicity correlation coefficient 0. CK, creatine kinase.Latest World News Videos. More Videos Watch Prince William and Kate video call students The Duke and Duchess of Cambridge made a video call with pupils and teachers at a school in the UK while the country is on lockdown.

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Ethnic Differences in Tissue Creatine Kinase Activity: An Observational Study

Latest international coronavirus videos Japan's Shinzo Abe promotes anti-flu drug to treat virus. Some world leaders ignore officials' warnings about CovidAs serum CK in healthy people is thought to occur from a proportional leak from normal tissues, we hypothesized that the black population subgroup has a generalized higher CK activity in tissues. We compared CK activity spectrophotometrically in tissues with high and fluctuating energy demands including cerebrum, cerebellum, heart, renal artery, and skeletal muscle, obtained post-mortem in black and white men.

We used mixed linear regression models to assess the possible influence of ethnicity on CK activity in different tissues, with ethnicity as a fixed categorical subject factor, and CK of different tissues clustered within one person as the repeated effect response variable. We collected post-mortem tissue samples from 17 white and 10 black males, mean age 62 y SE 4.

We found evidence that black people have higher CK activity in all tissues with high and fluctuating energy demands studied. This finding may help explain the higher serum CK levels found in this population subgroup. Furthermore, our data imply that there are differences in CK-dependent ATP buffer capacity in tissue between the black and the white population subgroup, which may become apparent with high energy demands.

Creatine kinase CK activity in serum is widely used to diagnose tissue damage including myocardial infarction and skeletal muscle myopathy, but it is unknown why serum CK activity is higher in apparently healthy black people of sub-Saharan African descent [1] — [4]. There is no evidence of muscle damage in black people as cause for the high serum CK activity, and the BB, MB, and MM isoenzymes in serum are proportionally higher, but have a normal distribution [2][4].

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Therefore, it was proposed that the black population subgroup has a generalized high CK activity in tissues, but hitherto there were no data to substantiate this [3]. In this paper, we report ethnic differences in CK activity in tissues with high and fluctuating energy demands. Tissues were collected in accordance with ethical approval of the accredited Medical Ethics Committee of the Academic Medical Center, Amsterdam, the Netherlands, whereby post-mortem small sample tissue collection was permitted with written consent from the donor, or a legal representative next-of-kin or other authorized representative.

We only included tissues of subjects presented within 30 h after death. Exclusion criteria included known HIV, hepatitis B, or C infection; Creutzfeldt-Jacob disease; chronic heart failure; myocardial infarction; brain infarction; or any widespread disease of the sampled organs.

Classification in black of sub-Saharan Africa or of sub-Saharan African descent, or white European was final when consensus was reached by at least two health workers pathologists, a member of the study group, or pathology assistants. Around 1 cubic centimeter of macroscopically healthy cerebral cortex gyrus precentraliscerebellar cortex, left cardiac ventricle, renal artery, and iliopsoas muscle was sampled.

The investigator was blinded for the ethnicity of the subjects. CK activity was determined according to Scholte et al. The baseline reaction rate was spectrophotometrically assessed Cary 1E Spectrophotometer; Varian Inc. The reaction rate was corrected for the baseline rate, and CK activity was calculated using a molar extinction coefficient of NADH of 6. The primary outcome was a difference in mean tissue creatine kinase activity between black and white people.

Correlations with tissue CK activity were calculated using Pearson's correlations coefficients, for storage time, ethnicity, and for age nested within ethnicity.He graduated with high honors in chemical engineering from University of Virginia.

Wo did a fellowship in adult gastroenterology at Emory University in Atlanta, Georgia. Wo also completed a research fellow in esophageal motility at Emory University. He has been the Associate Editor of the Diseases of the Esophagus since and served as a frequent research abstract reviewer for the Neurogastroenterology Section at the American Gastroenterological Association. Find a doctor. Faculty Search John M. Wo, MD. Phone Bio Dr. Gastroparesis, gastric electrical stimulation, small intestinal bacterial overgrowth.

Idiopathic gastroparesis is associated with specific transcriptional changes in the gastric muscularis externa. Most children with eosinophilic esophagitis have a favorable outcome as young adults. Narrow-band imaging versus white light for the detection of proximal colon serrated lesions: a randomized, controlled trial. How do gastroenterologists assess overall activity of eosinophilic esophagitis in adult patients?

video tang ck iu

Balloon expulsion testing for the diagnosis of dyssynergic defecation in women with chronic constipation. Therapeutic applications of ghrelin agonists in the treatment of gastroparesis.

Development and validation of a symptom-based activity index for adults with eosinophilic esophagitis. Gastric electrical stimulation with Enterra therapy improves symptoms of idiopathic gastroparesis.

Diagnosis and management of small intestinal bacterial overgrowth. A phase 2a, randomized, double-blind day study of TZP a ghrelin receptor agonist for diabetic gastroparesis. National survey of physicians' perception of the cause, complications, and management of gastroparesis. Randomised clinical trial: ghrelin agonist TZP relieves gastroparesis associated with severe nausea and vomiting--randomised clinical study subset data. Gastric electrical stimulation with Enterra therapy improves symptoms from diabetic gastroparesis in a prospective study.

Motility of the antroduodenum in healthy and gastroparetics characterized by wireless motility capsule. Effects of dexmedetomidine and propofol on lower esophageal sphincter and gastroesophageal pressure gradient in healthy volunteers. Pharmacokinetic profile of immediate-release omeprazole in patients with gastro-oesophageal reflux associated with gastroparesis. Current treatment of nausea and vomiting associated with gastroparesis: antiemetics, prokinetics, tricyclics.

Chemoprotective effects of Curcuma aromatica on esophageal carcinogenesis. A novel classification scheme for gastroparesis based on predominant-symptom presentation.

Treatment of older patients with hiatal hernia. Differences in intragastric pH in diabetic vs. Gastrointestinal and liver physiology Apr The use of dynamic positron emission tomography imaging for evaluating the carcinogenic progression of intestinal metaplasia to esophageal adenocarcinoma. Chemoprevention of carcinogenic progression to esophageal adenocarcinoma by the manganese superoxide dismutase supplementation.

Comparison of gastric emptying of a nondigestible capsule to a radio-labelled meal in healthy and gastroparetic subjects. Alterations in manganese superoxide dismutase expression in the progression from reflux esophagitis to esophageal adenocarcinoma. Exclusion of pH artifacts is essential for hypopharyngeal pH monitoring. Loss of manganese superoxide dismutase expression and activity in rat esophagus with external esophageal perfusion.

Double-blind, placebo-controlled trial with single-dose pantoprazole for laryngopharyngeal reflux.


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